Photo 51

O.1952-2

La « Photo 51 » est une image de diffraction aux rayons X dun gel paracristallin composé de fibres dADN. La photographie a fourni des informations essentielles pour déterminer la nature hélicoïdale des brins à double hélice de lADN.

https://www.letemps.ch/sciences/rosalind-franklin-chimiste-regarde-ladn

 

Photo 51 is an X-ray diffraction image of a paracrystalline gel composed of DNA fiber taken by Raymond Gosling, a graduate student working under the supervision of Rosalind Franklin in May 1952 at King’s College London, while working in Sir John Randall’s group. The image was tagged « photo 51 » because it was the 51st diffraction photograph that Franklin and Gosling had taken. It was critical evidence in identifying the structure of DNA.

James Watson was shown the photo by his collaborator, Maurice Wilkins, as Raymond Gosling, the author of the picture, had returned under his supervision. Rosalind Franklin did not know this at the time because she was leaving King’s College London. Randall, the head of the group, had asked Gosling to share all his data with Wilkins. Along with Francis Crick, Watson used characteristics and features of Photo 51, together with evidence from multiple other sources, to develop the chemical model of the DNA molecule. Their model, and manuscripts by Wilkins and colleagues, and Gosling and Franklin, were first published, together, in 1953, in the same issue of Nature. In 1962, the Nobel Prize in Physiology or Medicine was awarded to Watson, Crick and Wilkins. The prize was not awarded to Franklin; she had died four years earlier, and although there was not yet a rule against posthumous awards, the Nobel Committee generally does not make posthumous nominations. Likewise, Gosling’s work was not cited by the prize committee.

The photograph provided key information that was essential for developing a model of DNA. The diffraction pattern determined the helical nature of the double helix strands (antiparallel). The outside of the DNA chain has a backbone of alternating deoxyribose and phosphate moieties, and the base pairs, the order of which provides codes for protein building and thereby inheritance, are inside the helix. Watson and Crick’s calculations from Gosling and Franklin’s photography gave crucial parameters for the size and structure of the helix.

Photo 51 became a crucial data source that led to the development of the DNA model and confirmed the prior postulated double helical structure of DNA, which were presented in the series of three articles in the journal Nature in 1953.

As historians of science have re-examined the period during which this image was obtained, considerable controversy has arisen over both the significance of the contribution of this image to the work of Watson and Crick, as well as the methods by which they obtained the image. Franklin had been hired independently of Maurice Wilkins, who, taking over as Gosling’s new supervisor, showed Photo 51 to Watson and Crick without Franklin’s knowledge. Whether Franklin would have deduced the structure of DNA on her own, from her own data, had Watson and Crick not obtained Gosling’s image, is a hotly debated topic, made more controversial by the negative caricature of Franklin presented in the early chapters of Watson’s history of the research on DNA structure, The Double Helix. Watson admitted his distortion of Franklin in his book, noting in the epilogue: « Since my initial impressions about [Franklin], both scientific and personal (as recorded in the early pages of this book) were often wrong, I want to say something here about her achievements. »

Rosalind Franklin (May 1952) an X-ray photograph of B-DNA (called Photo 51)

http://archivesgamma.fr/1952/03/01/photo-51